A rapid method for the quantitative assay of proteolytic enzymes

✍ Scribed by Walter L. Nelson; Edward I. Ciaccio; George P. Hess

• Publisher: Elsevier Science
• Year: 1961
• Tongue: English
• Weight: 325 KB
• Volume: 2
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(61)90037-9

✦ Synopsis

Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured at various time intervals. Dye-protein complexes have been used since 1874 for the qualitative or semiquantitative determination of proteolytic enzymes (2-5). This paper describes a quantitative assay for proteolytic enzymes using insoluble dye-protein complexes and a convenient method for the preparation of stable substrates.

EXPERIMENTAL MATERIALS

Enzymes

Pepsin and trypsin were three-times recrystallized salt-free enzyme preparations obtained from Pentex, Inc .2 Pepsin, N.F. granular, was also used in some experiments.