Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured a
β¦ LIBER β¦
Biotinylated Enzyme Inhibitorsorbent Assay: A Specific Method for Quantitating Enzyme and Its Inhibitor
β Scribed by Zhibo Gan; Ronald R. Marquardt
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 101 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0003-2697
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## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europiumβtetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex