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Rapid Method for Quantitative Determination of Proteolytic Activity with Cyclic Voltammetry

✍ Scribed by Deniz Baş; İsmail Hakkı Boyacı

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
265 KB
Volume
22
Category
Article
ISSN
1040-0397

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A rapid method for the quantitative assa
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Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured a

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The paper describes an adaptation of the POPE and STEVENS method for a rapid assessment of proteolytic enzymes activity and protein hydrolysis intensity in raw materials of a marine origin. The adaptation involves substituting the titration by a fast colorimetric technique. A statistical treatment a