A sensitive assay for endo-β-N-acetylglucosaminidase H using a native glycoenzyme as substrate

A sensitive assay of galactocerebroside

A sensitive assay of galactocerebroside β-galactosidase by high-performance liquid chromatography using galactosyl-N-dansyl-sphingosine as substrate

✍ Makoto Naoi; Kunio Yagi 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 368 KB

A rapid and sensitive method was devised for determining &galactosidase activity specific for galactocerebroside. A fluorescent derivative of galactocerebroside, I-O-galactosyl-2-N-ldimethylaminonaphthalene-S-sulfonyl-sphingosine. was used as substrate, and the product, 2-N-l-dimethylaminonaphthal