✦ LIBER ✦

Assay of proteolytic enzymes by the fluorescence polarization technique

✍ Scribed by Hiroshi Maeda

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 439 KB
Volume: 92
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90649-3

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A rapid method for the quantitative assa

A rapid method for the quantitative assay of proteolytic enzymes

✍ Walter L. Nelson; Edward I. Ciaccio; George P. Hess 📂 Article 📅 1961 🏛 Elsevier Science 🌐 English ⚖ 325 KB

Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured a

Enzyme Assays by Fluorescence Polarizati

Enzyme Assays by Fluorescence Polarization in the Presence of Polyarginine: Study of Kinase, Phosphatase, and Protease Reactions

✍ Anton Simeonov; Xiahui Bi; Theo T. Nikiforov 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 94 KB

We have previously reported that the kinase catalyzed conversion of fluorescently labeled phosphate acceptor peptides to the corresponding phosphopeptides can be conveniently followed by measuring the fluorescence polarization signal in the presence of polyarginine. In the present work, we demonstra

Assay of follitropin and lutropin by flu

Assay of follitropin and lutropin by fluorescence enzyme immunoassay

✍ David A. Armbruster; F. Armbruster; Leah C. Hawes 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 438 KB

Recently developed fluorescent enzyme imrnunoassays (FEls) for follitropin (FSH) and lutropin (LH) designed for the Stratus analyzer were compared with a typical dual FSH-LH radioimmunoassay (RIA) procedure. The FEls use monoclonal antibodies in a sandwich reaction scheme similar to that of immunor

A sensitive microplate assay for the det

A sensitive microplate assay for the detection of proteolytic enzymes using radiolabeled gelatin

✍ B.D. Robertson; G.E. Kwan-Lim; R.M. Maizels 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 365 KB

A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By m

A Fluorescence Polarization Assay of Ery

A Fluorescence Polarization Assay of Erythrocyte Membrane Fluidity on the Abbott TDx Analyzer

✍ E.J. Palmer; P.S.M. Caines; R.J. Thibert; G.P. Brotea 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 280 KB

Detection of Phosphopeptides by Fluoresc

Detection of Phosphopeptides by Fluorescence Polarization in the Presence of Cationic Polyamino Acids: Application to Kinase Assays

✍ Jill Coffin; Maria Latev; Xiahui Bi; Theo T. Nikiforov 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 83 KB

We have studied the interaction of several phosphopeptides with cationic polyamino acids such as polyarginine and polylysine by fluorescence polarization. The phosphopeptides used were labeled with fluorescein, and their net charges at the experimental pH of 7.5 were 0, ؊1, ؊2, and ؊3. These phospho