Assay of follitropin and lutropin by fluorescence enzyme immunoassay

✍ Scribed by David A. Armbruster; F. Armbruster; Leah C. Hawes

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 438 KB
• Volume: 4
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860040304

✦ Synopsis

Recently developed fluorescent enzyme imrnunoassays (FEls) for follitropin (FSH) and lutropin (LH) designed for the Stratus analyzer were compared with a typical dual FSH-LH radioimmunoassay (RIA) procedure.

The FEls use monoclonal antibodies in a sandwich reaction scheme similar to that of immunoradiometric procedures but with filter paper tabs rather than polystyrene beads and alkaline phosphatase as a tag instead of a radioisotope. Precision of the FEI was comparable with that of the RIA tests with CVs typically under 10%. The detection limit of the FSH test is 0.4 lU1L and that of the LH test is 0.6 IUIL. Measurable carryover exists but is clinically inconsequential. Neither assay exhibits total specificity, but the degree of cross-reactivity is unlikely to be problematic in most situations. Recovery with the FSH assay was 103%, with the LH assay, it averaged 108%. Moderate amounts of bilirubin and triglycerides and gross hemolysis caused no significant interferences. Method comparison studies yielded the following: FEI for FSH, 1.16RIA+ 3 . 1 , r = 0 . 9 7 , n = 114;FEIfor LH, 0.77 RIA -0.23, r = 0.96, n = 112. Difference analysis of the method comparison data revealed relatively poor agreement between the FEI tests and the dual RIA procedure. With the adoption of method-specific normal reference ranges, the FEI tests offer attractive alternatives to radioisotopic FSH and LH assays.