Assay of proteolytic enzyme activity using a 14C-labeled hemoglobin
โ Scribed by Jay S. Roth; Thomas Losty; Eugen Wierbicki
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 385 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By m
Indel oxazi ne hydrochloride, (f ) -2-[( i nden-7-yl oxy)methyl]morphol ine hydrochloride (YM-08054), a new cerebral activator, was labeled w i t h 14C f o r metabolic studies. The labeled material with a s p e c i f i c a c t i v i t y o f 14.9 mCi/mmol was synthesized i n a 42.8% radiochemical y i
548) has been modified by using [3H]ATP to produce [3H]cyclic AMP and monitoring the recovery with [14C]cyclic AMP. The modified assay remains sensitive and accurate, but avoids the inconvenience and expense of using 32p.