The reversible formation of dithiourethanes from fluorescein isothiocyanate and mercaptoethanol or mercaptoethylamine has been studied. It is shown that it is possible to use the reaction as the basis for a spectrophotometric titration of a number of isothiocyanates with mercaptoethanol.
A simple method for determining the labeling efficiency of fluorescein isothiocyanate products
β Scribed by Roger M. McKinney; Janet T. Spillane; George W. Pearce
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 494 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0003-2697
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