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Excluded volume effects on the rate of renaturation of DNA

✍ Scribed by James G. Wetmur

Publisher: Wiley (John Wiley & Sons)
Year: 1971
Tongue: English
Weight: 529 KB
Volume: 10
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360100402

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📜 SIMILAR VOLUMES

Effects of microscopic and macroscopic v

Effects of microscopic and macroscopic viscosity on the rate of renaturation of DNA

✍ Chiang-Tung Chang; Timothy C. Hain; James R. Hutton; James G. Wetmur 📂 Article 📅 1974 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 558 KB

## Abstract The effect of solvent viscosity on DNA renaturation rates has been investigated as a function of temperature for a number of solvent systems. The results are all consistent with a microscopic viscosity limitation of the rate determining step. Rates of renaturation in perchlorate and qua

Effects of Na+ on the persistence length

Effects of Na+ on the persistence length and excluded volume of T7 bacteriophage DNA

✍ E. S. Sobel; J. A. Harpst 📂 Article 📅 1991 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 475 KB 👁 2 views

Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na+ concentration (0.005 to 3.0 M). All parameters decrease sharply as "a+] increases, and tend

One hundred-fold acceleration of DNA ren

One hundred-fold acceleration of DNA renaturation rates in solution

✍ Robert Wieder; James G. Wetmur 📂 Article 📅 1981 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 600 KB

## Abstract Solvents which accelerate DNA renaturation rates have been investigated. Addition of NaCl or LiCl to DNA in 2.4__M__ Et~4~NCl initially increases renaturation rates at 45°C and then leads to a loss of second‐order behavior. The greatest accelerations are seen with LiCl and dilute DNA. V

Excluded-volume effect on the bidimensio

Excluded-volume effect on the bidimensional conformation of DNA molecules adsorbed to protein films

✍ C. Frontali 📂 Article 📅 1988 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 173 KB 👁 1 views

me use of electron microscopic images to extract information concerning the parameters that characterize the flexibility of linear polymers in solution has been described by several authors.'-4 These attempts clearly rely on the hypothesis that interaction with the supporting substrate does not alte

Thermal stability and renaturation of DN

Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: Acceleration of the renaturation rate

✍ June F. Escara; James R. Hutton 📂 Article 📅 1980 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 682 KB

## Abstract The thermal stability and renaturation kinetics of DNA have been studied as a function of dimethyl sulfoxide (DMSO) concentration. Increasing the concentration of DMSO lowers the melting temperature of DNA but results in an increased second‐order renaturation rate. For example, in a DNA

Determination of the rate of renaturatio

Determination of the rate of renaturation of modified DNA by fluorescence depolarization

✍ Stephen J. Miller; James G. Wetmur 📂 Article 📅 1974 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 326 KB 👁 1 views

## Abstract Fluorescence depolarization was used to measure the rate of renaturation of T2 DNA, which had been modified by chloroacetaldehyde. Rates were measured on DNA samples with 5–15% of the base pairs modified and were found to agree with rates determined by DNA absorbance kinetics at 260 nm.