Excluded-volume effect on the bidimensional conformation of DNA molecules adsorbed to protein films
✍ Scribed by C. Frontali
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1988
- Tongue
- English
- Weight
- 173 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
me use of electron microscopic images to extract information concerning the parameters that characterize the flexibility of linear polymers in solution has been described by several authors.'-4 These attempts clearly rely on the hypothesis that interaction with the supporting substrate does not alter the flexibility of the chain molecule and or. an interpretation of the process by which the three-dimensional chain is brought to adhere to the bidimensional substrate.
Through the analysis of local curvatures assumed by DNA molecules adsorbed to cytochrome c films, we were able to obtain2 an absolute measure of the microscopic persistence length amicro. The relation of this local parameter, which reflects short-range interactions, to the Kratky and Porod persistence length, related to average molecular dimensions, was further discussed in a subsequent work? In this work it was shown that the two parameters coincide in the range of contour lengths between .5amicro and 3%TliCrO.