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Determination of the rate of renaturation of modified DNA by fluorescence depolarization

✍ Scribed by Stephen J. Miller; James G. Wetmur

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
326 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Fluorescence depolarization was used to measure the rate of renaturation of T2 DNA, which had been modified by chloroacetaldehyde. Rates were measured on DNA samples with 5–15% of the base pairs modified and were found to agree with rates determined by DNA absorbance kinetics at 260 nm. The renaturation rate of a modified T2 DNA was unchanged in the presence of a ninefold abundance of unlabeled calf thymus DNA.

πŸ“œ SIMILAR VOLUMES

Influence of magnesium ions on helix–coi
Influence of magnesium ions on helix–coil transition of DNA determined by modified differential scanning calorimeter
✍ Yoshihiro Baba; Akihiro Kagemoto πŸ“‚ Article πŸ“… 1974 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 301 KB

## Abstract The thermal effect of magnesium ions on the helix–coil transition of DNA was studied calorimetrically by a modified differential scanning calorimeter (DSC). It was found that the transition temperature of DNA depends on both the DNA and magnesium ion concentrations. The dependence of th