Electrophoretic mobilities of DNA molecules ranging in length from 200 to 48 502 base pairs (bp) were measured in agarose gels with concentrations T = 0.5% to 1.3% at electric fields from E = 0.71 to 5.0 V/cm. This broad data set determines a range of conditions over which the new interpolation equa
โฆ LIBER โฆ
Effects of Na+ on the persistence length and excluded volume of T7 bacteriophage DNA
โ Scribed by E. S. Sobel; J. A. Harpst
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1991
- Tongue
- English
- Weight
- 475 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0006-3525
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โฆ Synopsis
Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na+ concentration (0.005 to 3.0 M). All parameters decrease sharply as "a+] increases, and tend to level off at high Na' . The variation of persistence length with [ Na+] is consistent with predictions from counterion condensation theory.
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