Evolution of an acylase active on cephalosporin C
โ Scribed by Loredano Pollegioni; Simona Lorenzi; Elena Rosini; Giorgia Letizia Marcone; Gianluca Molla; Roberto Verga; Walter Cabri; Mirella S. Pilone
- Book ID
- 111752570
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 2005
- Tongue
- English
- Weight
- 963 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0961-8368
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๐ SIMILAR VOLUMES
An extracellular glutaryl-7-ACA acylase producing strain of Bacillus sp. has been isolated from soil using a sensitive TLC based assay procedure. The wild type strain exhibited a titre of 5.6 Units/ml after about 40 h growth.
Amino acids altered the production and activities of cephalosporin C acylase and penicillin V acylase from Aeromonas species ACY 95 to a varying degree. DL-Tryptophan enhanced the cephalosporin C acylase formation by 222% while suppressed the penicillin V acylase formation by 68%. Cephalosporin C a