Mutational Analysis of a Key Residue in
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Linda G. Otten; Charles F. Sio; Almer M. van der Sloot; Robbert H. Cool; Wim J.
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Article
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2004
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John Wiley and Sons
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English
β 153 KB
beta-Lactam acylases are crucial for the synthesis of semisynthetic cephalosporins and penicillins. Unfortunately, there are no cephalosporin acylases known that can efficiently hydrolyse the amino-adipic side chain of Cephalosporin C. In a previous directed evolution experiment, residue Asn266 of t