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Modifying the substrate specificity of penicillin G acylase to cephalosporin acylase by mutating active-site residues

✍ Scribed by Bora Oh; Kyunggon Kim; Jungeun Park; Jongchul Yoon; Dohyun Han; Youngsoo Kim


Book ID
116288523
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
284 KB
Volume
319
Category
Article
ISSN
0006-291X

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Mutational Analysis of a Key Residue in
✍ Linda G. Otten; Charles F. Sio; Almer M. van der Sloot; Robbert H. Cool; Wim J. πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 153 KB

beta-Lactam acylases are crucial for the synthesis of semisynthetic cephalosporins and penicillins. Unfortunately, there are no cephalosporin acylases known that can efficiently hydrolyse the amino-adipic side chain of Cephalosporin C. In a previous directed evolution experiment, residue Asn266 of t