Effect of amino acids on the production and activities of cephalosporin C acylase and penicillin V acylase from Aeromonas species ACY 95
✍ Scribed by Bhagwant S. Dehpande; Sudha S. Ambedkar; Dr. Jaiprakash G. Shewale
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 196 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Amino acids altered the production and activities of cephalosporin C acylase and penicillin V acylase from Aeromonas species ACY 95 to a varying degree. DL-Tryptophan enhanced the cephalosporin C acylase formation by 222% while suppressed the penicillin V acylase formation by 68%.
Cephalosporin C acylase (CCA), the enzyme that deacylates the cephalosporin C to form 7-aminocephalosporanic acid (7-ACA) and alpha-amino adipic acid, holds promise to revamp the multi step protocols, either chemical or enzymatic, for the production of 7-ACA . Most of the enzymes referred in the literature as CCA are glutaryl acylases capable of hydrolysing, to some extent, cephalosporin C (KUMAR etal. 1993). Recently, we have described the formation of a CCA and a penicillin V acylase (PVA) by Aeromonas sp. ACY 95 . The CCA is higly specific for cephalosporin C and does not hydrolyse glutaryl 7-ACA. We report here the effect of supplementation of amino acids on the production and activities of CCA and PVA.