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Cephalosporin modification: An extracellular glutaryl-7-ACA acylase fromBacillussp.

โœ Scribed by V. C. Sonawane; R. S. Jolly; R. M. Vohra


Book ID
104645904
Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
179 KB
Volume
18
Category
Article
ISSN
0141-5492

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โœฆ Synopsis


An extracellular glutaryl-7-ACA acylase producing strain of Bacillus sp. has been isolated from soil using a sensitive TLC based assay procedure. The wild type strain exhibited a titre of 5.6 Units/ml after about 40 h growth.


๐Ÿ“œ SIMILAR VOLUMES


Characterization of an industrial biocat
โœ Daniela Monti; Giacomo Carrea; Sergio Riva; Eva Baldaro; Giovanni Frare ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 170 KB

A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optim