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Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases

✍ Scribed by Ichiro Aramori; Masao Fukagawa; Mana Tsumura; Morita Iwami; Hiroki Ono; Yosuke Ishitani; Hitoshi Kojo; Masanobu Kohsaka; Yoshio Ueda; Hiroshi Imanaka

Book ID
113396813
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
819 KB
Volume
73
Category
Article
ISSN
0922-338X

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Characterization of an industrial biocat
Characterization of an industrial biocatalyst: Immobilized glutaryl-7-ACA acylase
✍ Daniela Monti; Giacomo Carrea; Sergio Riva; Eva Baldaro; Giovanni Frare πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 170 KB

A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optim