Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase fromPseudomonassp.
โ Scribed by M. C. Chen; L. L. Chen; Y. H. Lee; C. Y. Wen; W. S. Chu
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 273 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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๐ SIMILAR VOLUMES
A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optim
An industrial glutaryl-7-aminocephalosporanic acid acylase (GAR) possesses a significant broad substrate specificity that crosses over the usual cephalosporanic skeleton. Enantioselective amidase and even esterase activities have been observed with all the glutarates of racemic substrates investigat