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Purification and stability of glutaryl-7-ACA acylase from pseudomonas sp.

โœ Scribed by Ezio Battistel; Daniele Bianchi; Rossella Bortolo; Lucia Bonoldi


Book ID
105608176
Publisher
Springer-Verlag
Year
1998
Tongue
English
Weight
857 KB
Volume
69
Category
Article
ISSN
0273-2289

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A batch of the immobilized industrial biocatalyst glutaryl-7-ACA acylase (GA), one of the two enzymes involved in the biotransformation of cephalosporin C (CefC) into 7-aminocephalosporanic acid (7-ACA), was characterized. K(m) value for glutaryl-7-ACA was 5 mM. Enzyme activity was found to be optim