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Escherichia coli relA strains as hosts for amplification of pBR322 plasmid DNA

✍ Scribed by Michael Hecker; Andreas Schroeter; Friedrich Mach


Book ID
118715297
Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
240 KB
Volume
29
Category
Article
ISSN
0378-1097

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πŸ“œ SIMILAR VOLUMES


Physiological studies on pBR 322 DNA amp
✍ Dr. M. Hecker; A. Schroeter; F. Mach πŸ“‚ Article πŸ“… 1986 πŸ› John Wiley and Sons 🌐 English βš– 349 KB

Amino acid limitation leads in E. coli relA cells which cannot synthesize guanosine tetraphosphate (ppGpp) under these conditions to an amplification of pBR 322 DNA. We previously proposed that ppGpp produced in E . coli relA+ cells subjected to amino acid limitation inhibits pBR 322 DNA replication

Amplification of pBR322 plasmid DNA in E
✍ Dr. K. H. Hofmann; P. Neubauer; Sabine Riethdorf; M. Hecker πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 298 KB

## Abstract Fermenter studies under batch and fed‐batch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP

Amplification of different ColEl plasmid
✍ Dr. A. Schroeter; S. Riethdorf; M. Hecker πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 233 KB

Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColEl related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation

Recombination-dependent recircularizatio
✍ Conley, E. C. ;Saunders, J. R. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 977 KB

Monomeric pBR322 DNA that had been linearized at its unique SalI site transformed wild-type Escherichia coli with 102 to 103 times less efficiency than CCC plasmid DNA. Dose-response experiments indicated that a single linear plasmid 'molecule' was sufficient to produce a transformant. Transformatio

Replication of pBR322 DNA in stringent a
✍ Hecker, Michael ;Schroeter, Andreas ;Mach, Friedrich πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 392 KB

Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated o