Amino acid limitation leads in E. coli relA cells which cannot synthesize guanosine tetraphosphate (ppGpp) under these conditions to an amplification of pBR 322 DNA. We previously proposed that ppGpp produced in E . coli relA+ cells subjected to amino acid limitation inhibits pBR 322 DNA replication
Escherichia coli relA strains as hosts for amplification of pBR322 plasmid DNA
β Scribed by Michael Hecker; Andreas Schroeter; Friedrich Mach
- Book ID
- 118715297
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 240 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0378-1097
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## Abstract Fermenter studies under batch and fedβbatch conditions were carried out to test the possibility of plasmid pBR322 production in large amounts by using __E. coli relA__ strains. High amplification rates of pBR322 plasmid DNA were observed in __E. coli__ CP79 (__relA__) and __E. coli__ CP
Amino acid starved cells of an E. coli relA strain accumulate a large amount of pBR322 plasmid DNA. In this study ColEl related plasmids of different copy number and size including a high copy number plasmid mutant of pBR322 were amplified in a relA strain of E. coli K-12 under amino acid limitation
Monomeric pBR322 DNA that had been linearized at its unique SalI site transformed wild-type Escherichia coli with 102 to 103 times less efficiency than CCC plasmid DNA. Dose-response experiments indicated that a single linear plasmid 'molecule' was sufficient to produce a transformant. Transformatio
Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA+:CP78; relA:CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated o