Enzyme immunoassay and characterization of pancreatic stone proteins in human urine

✍ Scribed by Noriyuki Tatemichi; Chikako Takahashi; Shinobu Hayakawa; Tetsuo Hayakawa; Tokimune Shibata; Motoji Kitagawa; Hiroshi Sobajima; Yasuyuki Nakae

• Book ID: 102878698
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 502 KB
• Volume: 7
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860070611

✦ Synopsis

Abstract

An enzyme immunoassay of pancreatic stone protein (PSP) in human urine was developed. Mean analytical recovery of pure PSP‐S~2‐5~ added to urine was 102.3% (SD 5.9%), and the precision of the assay was 2.0‐2.7% within an assay and 2.5‐2.9% between assays. In healthy volunteers (age 20‐55 years), the mean value of the PSP concentration, expressed as ratios to urine creatinine, was 129 ± 88 (mean ± SD) μg/g without any differences for sex. Urine PSP correlated with urine N‐acetylglucosaminidase (NAG) (r = 0.354). The molecular forms of immunoreactive PSP in urine were characterized by using cation exchange chromatography (Mono S), SDS‐PAGE, N‐terminal sequence, and enzyme immunoassay analysis. The urine PSP, eluted at the position corresponding to PSP‐S~2‐5~ on cation exchange chromatography, was converted to PSP‐S~1~ by trypsin digestion. The difference in mobility on SDSPAGE between urine PSP and PSP‐S~2‐5~ seems to be due to a glycosylated undecapeptide (N‐terminal 1‐11). The proposed method offers a sensitive, specific, and reproducible tool for laboratory analysis of human urine PSP levels. © 1993 Wiley‐Liss, Inc.