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Enzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum

✍ Scribed by Tomoyuki Katsuzaki; Noriyuki Tatemichi; Chikako Takeichi; Dr. Shinobu Hayakawa; Tetsuo Hayakawa; Tokimune Shibata; Yasuyuki Nakae; Satoru Naruse

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 414 KB
Volume: 6
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860060308

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✦ Synopsis

Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one-step enzyme immunoassay (EIA). PSP-S2-5 was employed as the standard in the assay. The assay's measurable range was 25-1,500 nglml and within run coefficient of variation was 3.7-6.4%. Analytical recovery of the assay was 101.5 ? 5.65% (X ? SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP-S2-5. The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis.

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