A highly sensitive enzyme immunoassay of anti-insulin antibodies in human serum

✍ Scribed by Takeyuki Kohno; Eiji Ishikawa; Satoru Sugiyama; Syuji Nakamura; Yoshimasa Kanemaru

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 431 KB
• Volume: 1
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860010205

✦ Synopsis

A highly sensitive enzyme immunoassay who had been treated with insulin for 0.6of anti-insulin antibodies in human serum 24 months. The detection limit of anti-inis described. Serum samples were sub-sulin IgG in human serum was 1,000 to jected to successive processes of the in-3,000-fold less than that obtained by the cubation with insulin, the dextran-charcoal previously reported enzyme immunoastreatment to remove free insulin, the pre-say, in which an insulin-coated polystyrene cipitation of insulin anti-insulin antibodies ball was incubated with diluted serum and by polyethylene glycol, the acid treatment subsequently with (antihuman IgG ?-chain) of the precipitates to inactivate anti-insulin Fab'-horseradish peroxidase conjugate.

antibodies, and the measurement of insu-The present enzyme immunoassay may be lin by sandwich enzyme immunoassay useful for the measurement of antibodies technique. By this enzyme immunoassay, for not only insulin but also other antigens anti-insulin antibodies were demonstrated that are not precipitated by polyethylene in most of serum samples from patients glycol.