In order to study the mechanism and origin of urine pancreatic stone protein (PSP), PSP was analyzed in the urine and sera from healthy subjects, patients with renal disease, and intensive care patients by Mono S chromatography and Western blotting. The elution patterns could be classified into thre
Nh2-Terminal sequence of novel pancreatic stone protein-related protein in human urine
โ Scribed by Masanori Kato; Noriyuki Tatemichi; Shinobu Hayakawa; Tetsuo Hayakawa
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 163 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0887-8013
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โฆ Synopsis
In a previous report, we detected the novel PSP-related protein in urine from a patient with diabetic nephropathy. This protein was different from PSP S1 and PSP S2-5 on the elution points of Mono S chromatography, To investigate the NH2-terminal sequence, we purified this protein by reverse phase chromatography and performed amino acid analysis. The results showed that the NHg-terminal sequence of this protein was one residue (arginine) longer than that of PSP S1. @ 1995 wiley-Liss, inc.
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