Enzyme immunoaassay for specific analysis of pancreatic stone proteins in human pancreatic juice

✍ Scribed by Tomoyuki Katsuzaki; Noriyuki Tatemichi; Chikako Takeichi; Dr. Shinobu Hayakawa; Tetsuo Hayakawa; Tokimune Shibata; Hideo Harada; Koji Ochi

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 341 KB
• Volume: 6
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860060607

✦ Synopsis

In order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-SI converted from PSP-S2-5 on PSP determination and the assay method for PSP-SI precipitate after solubilizing PSP-Sl. When bovine trypsin was added to pancreatic juice, PSP-S, was converted from PSP-S2-5 and precipitated about 45-85% after 1 h. The precipitated PSP-S1 was dissolved in 0.1 M sodium acetate buffer, pH 4.0, and the concentration was measured by the enzyme immunoassay, with similar reactivity to PSP-S, and PSP-S2-5. The proposed method can offer accurate and specific analysis of the PSP level in pancreatic juice. The results of the fractionation of pancreatic juice and duodenal juice on Mono S cation-exchange chromatography suggested that the major component of PSP was PSP-5 2 -5 in pancreatic juice and PSP-S, in duodenal juice. o 1992 Wiley-Liss, Inc.