Characterization in rat pancreatic juice of a protein homologous to the human pancreatic stone protein
✍ Scribed by Zygmunt Adrich; Alain M. De Caro; Andréa A. Guidoni; Mireille E. Woudstra; Mireille Rovery
- Book ID
- 113323009
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 578 KB
- Volume
- 93
- Category
- Article
- ISSN
- 1096-4959
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In order to study the concentration of pancreatic stone protein (PSP) in human pancreatic juice, we investigated the influence of the insoluble form of PSP-SI converted from PSP-S2-5 on PSP determination and the assay method for PSP-SI precipitate after solubilizing PSP-Sl. When bovine trypsin was a
In order to study the mechanism and origin of urine pancreatic stone protein (PSP), PSP was analyzed in the urine and sera from healthy subjects, patients with renal disease, and intensive care patients by Mono S chromatography and Western blotting. The elution patterns could be classified into thre
## Abstract An enzyme immunoassay of pancreatic stone protein (PSP) in human urine was developed. Mean analytical recovery of pure PSP‐S~2‐5~ added to urine was 102.3% (SD 5.9%), and the precision of the assay was 2.0‐2.7% within an assay and 2.5‐2.9% between assays. In healthy volunteers (age 20‐5