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Enzymatic synthesis of tritium — labelled prostaglandin D2 and its conversion to other prostaglandins

✍ Scribed by Stanislav I. Shram; Tatyana Yu. Lazurkina; Valerii P. Shevchenko; Igor Yu. Nagaev; Nikolai F. Myasoedov

Publisher
John Wiley and Sons
Year
1994
Tongue
French
Weight
361 KB
Volume
34
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

The one‐stage enzymatic synthesis of tritium‐labelled prostaglandin D~2~ from labelled arachidonic acid was performed by using the enzyme system PGH‐synthetase/PGH‐PGD‐isomerase. By enzymatic and chemical transformation of [^3^H]PGD~2~ the following compounds were obtained: 15‐keto‐13,14‐dihydro‐[^3^PGD~2~, 9α,11β‐[^3^H]PGD~2~, 9‐deoxy‐Δ^9^‐[^3^H]‐PGD~2~ ([^3^H]PGJ~2~) and Δ^12^‐13,14‐dihydro‐[^3^H]PGJ~2~. It was found that L‐selectride is a more effective reducing agent than sodium borohydride in the synthesis of ^9^α,11β‐[^3^H]PGF~2~.

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