A procedure for the determination of free and total cholesterol in lipid extracts is described. The method for free cholesterol employs cholesterol oxidase to generate H202 and peroxidase to catalyze the reaction of Hz02 with o-dianisidine to yield a colored product. For the determination of total
Enzymatic Quantitation of Cholesterol Esters in Lipid Extracts
β Scribed by Paul P. Van Veldhoven; Els Meyhi; Guy P. Mannaerts
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 98 KB
- Volume
- 258
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Selective production of emulsifiers from glucose and fatty acids has been achieved using an immobilized Candida antarctica lipase. Optimization of process selectivity considers the solubilities of the sugar and its monoesters in acetone at different temperatures, the percentage of this organic solve
Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, cholinecontaining phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal (n = 27), papillary cancer (n = 15), adenoma (n = 13) and Basedow diseas
The enzymatic synthesis of several sorbitol diesters whose HLB values are similar to those of monoglycerides (the largest single type of food-grade emulsifiers) has been studied. The procedure is carried out by the simple addition of the polyol to a solution of the fatty acid and is based on continu
## Determination of homocysteine levels in cells and serum is important because high homocysteine is a risk factor for cardiovascular disease. The currently used methods for homocysteine analysis either are time consuming or rely on the use of expensive equipment. Described in this study is an enz