𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A sensitive enzymatic assay for determination of cholesterol in lipid extracts

✍ Scribed by F.Omodeo Salè; S. Marchesini; P.H. Fishman; B. Berra

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
278 KB
Volume
142
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A procedure for the determination of free and total cholesterol in lipid extracts is described.

The method for free cholesterol employs cholesterol oxidase to generate H202 and peroxidase to catalyze the reaction of Hz02 with o-dianisidine to yield a colored product. For the determination of total cholesterol, cholesterol ester hydrolase is included. o 1984 Academic PW.

Inc.

📜 SIMILAR VOLUMES

A sensitive fluorometric assay for the d
A sensitive fluorometric assay for the determination of DNA
✍ Richard Vytášek 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 421 KB

A sensitive tluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described. Fluorescence is proportional to the amount of DNA over the range 0.05-5 pg of DNA when the reaction is conducted in a volume o

A sensitive assay for the determination
A sensitive assay for the determination of propionyl-CoA in biological material
✍ H.D. Söling; B. Volkmann 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 372 KB

BIOCi-IEMiSTRY 52, 30&310 (19%) A Sensitive Assay for the Determination of Propionyl-CoA in Biological Material Propionyl-CoA is a regular intermediate in the metabolism of the branched chain amino acid isoleucine in higher organisms and a metabolite in the catabolism of L-valine in Pseudomonas aeru

A sensitive spectrophotometric method fo
A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts
✍ Francesco Paoletti; Donatella Aldinucci; Alessandra Mocali; Anna Caparrini 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 475 KB

Superoxide dismutase (EC 1.15.1.1) has been assayed by a spectrophotometric method based on the inhibition of a superoxide-driven NADH oxidation. The assay consists of a purely chemical reaction sequence which involves EDTA, Mn(II), mercaptoethanol, and molecular oxygen, requiring neither auxiliary