Quantitation by 1H-NMR of dolichol, cholesterol and choline-containing lipids in extracts of normal and phathological thyroid tissue
✍ Scribed by Yoshichika Yoshioka; Jun Sasaki; Masahide Yamamoto; Kazuyoshi Saitoh; Shigeyuki Nakaya; Manabu Kubokawa
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 95 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0952-3480
No coin nor oath required. For personal study only.
✦ Synopsis
Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, cholinecontaining phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal (n = 27), papillary cancer (n = 15), adenoma (n = 13) and Basedow disease (n = 6)]. In normal thyroid the mean concentrations of dolichol, cholesterol and phospholipids were 1.2, 3.6 and 2.1 mmol/g wet weight, respectively. The concentrations of these lipids exhibited positive mutual correlations and positive correlations with patient age. The increase in dolichol in elderly human thyroid may be due to the accumulation of lysosomes and may help to compensate for the decrease in the activity of lysosomal enzymes and in thyroid hormone production and release. Dolichol concentrations were significantly lower in papillary cancer (0.4 mmol/g) and Basedow disease (0.3 mmol/g) compared to normal thyroid (p 0.01 and p 0.05, respectively), while cholesterol was enhanced only in cancer tissue (10.7 mmol/g). Benign adenoma exhibited normal levels of both dolichol and cholesterol. These results suggest that the synthesis and accumulation of isoprenoids are normal in adenoma but not in cancer.