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Enzymatic method for the determination of tellurite ions

✍ Scribed by Dai-Eun Sok; Mee Ree Kim

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 554 KB
Volume: 298
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(94)00289-4

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✦ Synopsis

A simple method for the quantitative determination of tellurite ions in aqueous media including blood plasma or serum is described. This assay is based on the selective and potent inhibition of a Zn*+ -glycerophosphocholine phosphocholine phosphodiesterase by tellurite ions in the presence of tetramethylammonium ions. The assay method, which showed a good relationship between the enzyme inhibition and the amount of tellurite ions (50 nM-5 PM), was found to be rapid (< 5 min), reproducible (mean C.V., 6.5%), and at the same time, sensitive, showing a low limit of detection (50 nmol). Based on this technique, the removal of tellurites by thiols, and the kinetics for the uptake of tellurite ions in blood were investigated.

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