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Enzymatic method for determination of the degree of deacetylation of chitosan

✍ Scribed by Fumio Nanjo; Ryosuke Katsumi; Kazuo Sakai

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
363 KB
Volume
193
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new method was developed for the accurate determination of the degree of deacetylation of chitosan. The method involves the complete hydrolysis of chitosan to glucosamine and N-acetylglucosamine by a cooperative action of chitosanolytic enzymes exo-beta-D-glucosaminidase, beta-N-acetylhexosaminidase, and chitosanase, and subsequent determination of the monosaccharides by specific colorimetric assays or HPLC. The conditions required for the complete hydrolysis of chitosan were examined and the degree of deacetylation of several chitosan samples was determined.

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✍ Jian Yang; Feng Tian; Zheng Wang; Qing Wang; Yan-Jun Zeng; Shi-Qian Chen πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 187 KB

## Abstract Comparative studies have been carried out among solid‐state chitosan soliquoid, chitosan acetic acid physiological saline solution, and carboxymethyl chitosan physiological saline solution to discover the hemostatic effect of molecular weight (__M__~w~) and deacetylation degree (DA) of