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Method for enzymatic determination of imidazole acetic acid

โœ Scribed by Takehiko Watanabe; Hiroshi Kambe; Ikuo Imamura; Yoshitaka Taguchi; Toshihide Tamura; Hiroshi Wada

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
559 KB
Volume
130
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for enzymatic assay of imidazole acetic acid (ImAA) was developed, based on the strict substrate specificity of imidazole acetate monooxygenase from Pseudomonas sp. [Maki et al. (1969) J. Biol. Chem., 244., 2942-29501, which catalyzes concomitant conversion of NADH to NAD+. Thus, ImAA was determined by measuring decrease in absorbancy at 340 nm. Tissue extracts were partially purified and/or concentrated by column chromatography on Bio-Rad AC-1 before enzymatic assay. The lowest measurable level of ImAA by this method was 2 nmol.

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