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An enzymatic method for the determination of dCMP in picomol amounts

✍ Scribed by Kerstin Brynolf

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
578 KB
Volume
72
Category
Article
ISSN
0003-2697

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✦ Synopsis

An enzymatic method for the determination of picomol amounts of dCMP in cellfree extracts was developed. The method is based on the quantitative phosphorylation of dCMP to dCTP by a partially purified dCMP-kinase and a pure NDP-kinase.

The dCTP formed is measured using a DNApolymerase assay. With this technique as little as 10 pmoles of dCMP can be measured accurately. The method was applied to the study of dCMP, dCDP, and dCTP pools in resting and growing cells. * Abbreviations and enzymes: NDP-kinase nucleoside diphospho kinase (EC 2.7.4.6); dCMP-kinase (EC 2.7.3.14); DNA-polymerase (EC 2.7.7.7).

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An enzymatic method for measuring picomole quantities of acetylcholine and choline in CNS tissue
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A rapid and sensitive enzymatic assay for measuring picomole quantities of both acetylcholine (ACh) and choline (Ch) in tissue extracts has been developed. After ACh and Ch were extracted into 15% 1 N formic acid/85% acetone by the procedure of Toru and Aprison, lipids were removed by a heptane-chlo