Method of enzymatic determination of pyrroloquinoline quinone

✍ Scribed by Minoru Ameyama; Masatsugu Nonobe; Emiko Shinagawa; Kazunobu Matsushita; Osao Adachi

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 415 KB
• Volume: 151
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90174-5

✦ Synopsis

An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which ~-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous pyrroloquinoline quinone in the presence of magnesium ions gave a quantitative determination of pyrroloquinoline quinone by assaying the restored Dglucose dehydrogenase activity. This novel enzymatic method was confirmed to be highly reproducible up to 10 ng of pyrroloquinoline quinone and could be applied to a routine assay of pyrroloquinoline quinone. Q 1985 Academic Pres, Inc.