Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the
Enrichment of plasmid DNA in cell membranes of Bacillus subtilis
β Scribed by Kerstin Nyberg
- Book ID
- 109312929
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 419 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0378-1097
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A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra
When plasmids carrying leucine genes of Bacillus subtilis 168 were isolated from a restriction and modification deficient (r-m-) strain and used for transformation of a restricting strain B. subtilis 168 leu recE4, the number of transformants was greatly reduced. Transformation of rec+ strain (trans