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Integration and excision of a plasmid in Bacillus subtilis

✍ Scribed by Galizzi, A. ;Scoffone, F. ;Milanesi, G. ;Albertini, A. M.


Publisher
Springer
Year
1981
Tongue
English
Weight
693 KB
Volume
182
Category
Article
ISSN
0026-8925

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A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived

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Two HindIII generated DNA fragments of 3.0 and 2.3 Kb derived from rRNA genes of B. subtilis were cloned in E. coli with pBR322. The 3.0 Kb fragment could be subcloned in B. subtilis using pC194. However, only the multimeric, but not the monomeric derivatives of this hybrid plasmid were active in tr

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