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Plasmid transformation in Bacillus subtilis

✍ Scribed by Bensi, G. ;Iglesias, A. ;Canosi, U. ;Trautner, T. A.

Publisher: Springer
Year: 1981
Tongue: English
Weight: 859 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00352512

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✦ Synopsis

A series of hybrid plasmids consisting of pC194 or pUBll2 and B. subtilis DNA were constructed. In contrast to plasmid pC 194, purified monomeric forms of such plasmids were active in transformation, provided the recipient cells were recombination proficient. Similarly the monomers of pC194 derived plasmids, containing bacteriophage dpl05 DNA were able to transform d)105 lysogenic but not nonylsogenic cells. From the results it is concluded that the presence of DNA/DNA homology between chromosomal DNA of the recipient cell and part of the hybrid plasmids used is a sufficient condition to endow monomeric plasmids with transforming activity. * This work is part of the Doctoral Thesis to be submitted by A.

Iglesias to the Freie Universitfit Berlin demonstrate the generality of the activation phenomenon reported with pBC1.

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