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Restriction of plasmid-mediated transformation in Bacillus subtilis 168

✍ Scribed by Tanaka, Teruo

Publisher: Springer
Year: 1979
Tongue: English
Weight: 239 KB
Volume: 175
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425542

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✦ Synopsis

When plasmids carrying leucine genes of Bacillus subtilis 168 were isolated from a restriction and modification deficient (r-m-) strain and used for transformation of a restricting strain B. subtilis 168 leu recE4, the number of transformants was greatly reduced. Transformation of rec+ strain (transformation by integration of the donor DNA into the chromosome) with the plasmids was not affected irrespective of whether the recipient carried the r+ or r- phenotype. These results show that the plasmid-mediated transformation is subject to the host controlled restriction and suggest that r-m- strains should be used for construction of recombinant DNA molecules in B. subtilis 168.

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