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Restriction and modification in Bacillus subtilis 168

✍ Scribed by Fučík, Vladimir ;Grünnerová, Helena ;Zadražil, Stanislav

Publisher: Springer
Year: 1982
Tongue: English
Weight: 423 KB
Volume: 186
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00422922

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✦ Synopsis

Gene hsrM (nonB) of Bacillus subtilis 168, causing non-permissiveness to phage SP10 (Saito et al. 1979) and reduced plating efficiency of unmodified phage ~)105, is responsible for non-permissiveness of B. subtilis 168 for phages #15 and PZA. Upon transformation to sporulation deficiency (allele spoOA) B. subtilis 168 becomes permissive for qbl5 and PZA and loses the ability to restrict #105. spoOA str-1 double transformants of B. subtilis 168, however, retain the restriction 168 and non-permissiveness for ~)15 and PZA phages, in spite of their Spo-phenotype. Therefore it appears that a functional product of the spoOA gene is required for expression of gene hsrM in wild-type bacteria, but is not essential in streptomycin-resistant bacteria. Phage genomes (PZA) were trapped in spores of the restriction deficient strain with much higher efficiency than in the wild-type.

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