Gene hsrM (nonB) of Bacillus subtilis 168, causing non-permissiveness to phage SP10 (Saito et al. 1979) and reduced plating efficiency of unmodified phage ~)105, is responsible for non-permissiveness of B. subtilis 168 for phages #15 and PZA. Upon transformation to sporulation deficiency (allele spo
Restriction and modification in Bacillus subtilis
✍ Scribed by Arwert, Fré ;Rutberg, Lars
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 160 KB
- Volume
- 133
- Category
- Article
- ISSN
- 0026-8925
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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h
A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperate Bacillus subtilis phage SPfi. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence