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Restriction and modification in Bacillus subtilis

✍ Scribed by Arwert, Fré ;Rutberg, Lars


Publisher
Springer
Year
1974
Tongue
English
Weight
160 KB
Volume
133
Category
Article
ISSN
0026-8925

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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h

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A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperate Bacillus subtilis phage SPfi. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence