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Restriction and modification in Bacillus subtilis: Identification of a gene in the temperate phage SPβ coding for a BsuR specific modification methyltransferase

✍ Scribed by Trautner, T. A. ;Pawlek, B. ;Günthert, U. ;Canosi, U. ;Jentsch, S. ;Freund, M.

Publisher
Springer
Year
1980
Tongue
English
Weight
731 KB
Volume
180
Category
Article
ISSN
0026-8925

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✦ Synopsis

A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperate Bacillus subtilis phage SPfi. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SPfi DNA as well as in heterologous DNA.

📜 SIMILAR VOLUMES

Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
✍ Montenegro, M. A. ;Pawlek, B. ;Behrens, B. ;Trautner, T. A. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 451 KB

Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h