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Host controlled modification and restriction in Bacillus subtilis

✍ Scribed by Shibata, Takehiko ;Ando, Tadahiko


Publisher
Springer
Year
1974
Tongue
English
Weight
325 KB
Volume
131
Category
Article
ISSN
0026-8925

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πŸ“œ SIMILAR VOLUMES


Host-controlled modification and restric
✍ Ikawa, Shukuko ;Shibata, Takehiko ;Ando, Tadahiko ;Saito, Hiuga πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 412 KB

A Bsu168-specific restriction deficient (r168-) mutant of Bacillus subtilis Marburg 168 was transformed to be BsuR-specific restriction proficient (rR+) with B. subtilis R DNA as efficiently as the Bsu 168-specific restriction proficient (r168+) parental strain (hsrM+, hsdR-). We constructed rR+ mR+

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Gene hsrM (nonB) of Bacillus subtilis 168, causing non-permissiveness to phage SP10 (Saito et al. 1979) and reduced plating efficiency of unmodified phage ~)105, is responsible for non-permissiveness of B. subtilis 168 for phages #15 and PZA. Upon transformation to sporulation deficiency (allele spo

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Bacillus amyloliquefaciens N produces two restriction enzymes, BamNI and BamNx. Subtilis-phage phi 1 is strongly restricted by BamNx. We isolated phi 1 rH, a mutant of phage phi 1, which overcame the BamNx-restriction by producing inhibitor. This inhibitor inactivated BamNx specifically and reversib

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Restriction and modification in B. subti
✍ Bron, Sierd ;Luxen, Erik ;Venema, Gerard ;Trautner, Thomas A. πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 931 KB

The effects of restriction in vivo by competent B. subtilis R cells and in vitro by purified endonuclease BsuR on transformation and transfection with native and denatured DNA were investigated. The results show that transformation by either native, or denatured DNA is not affected by restriction, w