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Restriction and modification in B. subtilis

✍ Scribed by Bron, Sierd ;Luxen, Erik ;Venema, Gerard ;Trautner, Thomas A.

Publisher
Springer
Year
1980
Tongue
English
Weight
931 KB
Volume
179
Category
Article
ISSN
0026-8925

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✦ Synopsis

The effects of restriction in vivo by competent B. subtilis R cells and in vitro by purified endonuclease BsuR on transformation and transfection with native and denatured DNA were investigated. The results show that transformation by either native, or denatured DNA is not affected by restriction, whereas transfection both with native and denatured SPP1 DNA is severely restricted. In contrast to the results obtained in vivo, the biological activity of native and denatured transforming DNA is destroyed by BsuR in vitro, as is the transfecting activity of native and denatured SPP1 DNA. The sensitivity of denatured DNA, either with mixtures of the complementary strands or with separated single strands alone, is significantly lower than that of native DNA. The results are discussed in the context of possible mechanisms underlying the different responses of transforming and transfecting DNA to in vivo restriction by B. subtilis R cells.

πŸ“œ SIMILAR VOLUMES

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ Trautner, Thomas A. ;Pawlek, Brigitte ;Bron, Sierd ;Anagnostopoulos, Constantine πŸ“‚ Article πŸ“… 1974 πŸ› Springer 🌐 English βš– 665 KB
Restriction and modification in B. subti
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✍ Bron, Sierd ;Luxen, Erik ;Trautner, Thomas A. πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 611 KB

Non-modified DNAs from phages SPO2 and phi 105, and prophage DNAs extracted from lysogens carrying these phages, were used to transfect isogenic r+m+ B. subtilis recipients which were either non-lysogenic, or had been lysogenized with a homologous or a non-homologous phage. Restriction of transfecti

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ Bron, Sierd ;Murray, Kenneth πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 739 KB

Restriction endonuclease R from Bacillus subtilis strain R cleaves nonmodified SPP 1 DNA in approximately 80, and lambda DNA in about 200 different sites. DNA digests with this endonuclease and with endonuclease Hae III from Haemophilus aegyptius show identical fragmentation patterns on gel electrop

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ GΓΌnthert, U. ;Stutz, J. ;Klotz, G. πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 440 KB

The content of 5-methylcy~osine (SMC) and 6-methyladenine (6MA) in modified and nonmodified DNAs from B. subtilis and B. subtilis phage SPP1 were determined. Nonmodified SPP1.0 DNA contains about 15 5~C residues/molecule. Each modified SPP1 .R DNA molecule carries 190 modification specific methyl gr

Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
✍ Montenegro, M. A. ;Pawlek, B. ;Behrens, B. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 451 KB

Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h