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Restriction and modification in B. subtilis

✍ Scribed by Günthert, U. ;Stutz, J. ;Klotz, G.

Publisher
Springer
Year
1975
Tongue
English
Weight
440 KB
Volume
142
Category
Article
ISSN
0026-8925

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✦ Synopsis

The content of 5-methylcy~osine (SMC) and 6-methyladenine (6MA) in modified and nonmodified DNAs from B. subtilis and B. subtilis phage SPP1 were determined. Nonmodified SPP1.0 DNA contains about 15 5~C residues/molecule. Each modified SPP1 .R DNA molecule carries 190 modification specific methyl groups. This number is sufficient to account for modification of the 80 restriction sites in SPP1 DNA (Bron and Murray, 1975) against endo 1%. Bsu 1%, assuming each modified site contains two 5MC residues. Resistance of SPO1 DNA against endo R. Bsu R restriction both in vivo and in vitro is probably not due to methylation of endo R.Bsu R recognition sites.

Biological parameters of a restriction system of B. subtilis were described in a previous communication (Trautner etal., 1974). Subsequently, a restriction endonuclease--endo R. Bsu R was isolated and purified from restricting strains of B. subtilis (Bron et al., 1975). This enzyme recognizes and cuts (indicated by arrows) the following nucleotide sequence (Bron and Murray, 1975):

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