𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Restriction and modification in B. subtilis

✍ Scribed by Bron, Sierd ;Murray, Kenneth

Publisher
Springer
Year
1975
Tongue
English
Weight
739 KB
Volume
143
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Restriction endonuclease R from Bacillus subtilis strain R cleaves nonmodified SPP 1 DNA in approximately 80, and lambda DNA in about 200 different sites. DNA digests with this endonuclease and with endonuclease Hae III from Haemophilus aegyptius show identical fragmentation patterns on gel electrophoresis, indicating that the two enzymes recognise the same nucleotide sequence. The polynucleotide kinase reaction was used in conjunction with two-dimensional ionophoretic nucleotide mapping methods to identify the 5'-nucleotide sequences at the sites of cleavage by the B. subtilis restriction endonuclease. The results show that the recognition sequence is (see article) where arrows indicate the points of strand scission. Each of the four possible nucleotides can occur in the positions flanking the recognition site.

πŸ“œ SIMILAR VOLUMES

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ Trautner, Thomas A. ;Pawlek, Brigitte ;Bron, Sierd ;Anagnostopoulos, Constantine πŸ“‚ Article πŸ“… 1974 πŸ› Springer 🌐 English βš– 665 KB
Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ Bron, Sierd ;Luxen, Erik ;Venema, Gerard ;Trautner, Thomas A. πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 931 KB

The effects of restriction in vivo by competent B. subtilis R cells and in vitro by purified endonuclease BsuR on transformation and transfection with native and denatured DNA were investigated. The results show that transformation by either native, or denatured DNA is not affected by restriction, w

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ Bron, Sierd ;Luxen, Erik ;Trautner, Thomas A. πŸ“‚ Article πŸ“… 1980 πŸ› Springer 🌐 English βš– 611 KB

Non-modified DNAs from phages SPO2 and phi 105, and prophage DNAs extracted from lysogens carrying these phages, were used to transfect isogenic r+m+ B. subtilis recipients which were either non-lysogenic, or had been lysogenized with a homologous or a non-homologous phage. Restriction of transfecti

Restriction and modification in B. subti
Restriction and modification in B. subtilis
✍ GΓΌnthert, U. ;Stutz, J. ;Klotz, G. πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 440 KB

The content of 5-methylcy~osine (SMC) and 6-methyladenine (6MA) in modified and nonmodified DNAs from B. subtilis and B. subtilis phage SPP1 were determined. Nonmodified SPP1.0 DNA contains about 15 5~C residues/molecule. Each modified SPP1 .R DNA molecule carries 190 modification specific methyl gr

Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
✍ Montenegro, M. A. ;Pawlek, B. ;Behrens, B. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1983 πŸ› Springer 🌐 English βš– 451 KB

Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h