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Restriction and modification in B. subtilis

✍ Scribed by Bron, Sierd ;Luxen, Erik ;Trautner, Thomas A.

Publisher
Springer
Year
1980
Tongue
English
Weight
611 KB
Volume
179
Category
Article
ISSN
0026-8925

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✦ Synopsis

Non-modified DNAs from phages SPO2 and phi 105, and prophage DNAs extracted from lysogens carrying these phages, were used to transfect isogenic r+m+ B. subtilis recipients which were either non-lysogenic, or had been lysogenized with a homologous or a non-homologous phage. Restriction of transfecting phage and prophage DNA occurred in non-lysogenic recipients and in recipients lysogenic for a non-homologous phage. No effect of restriction was observed when phage or prophage DNA was used to transfect recipients carrying a homologous prophage. This is analogous to the absence of restriction in transformation and indicates that in B. subtilis the distinction between transforming and transforming and transfecting DNA is not made at the initial stages of DNA uptake and processing, but rather at later stages, where recognition of homologous regions in donor and recipient DNA plays an important role.

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The content of 5-methylcy~osine (SMC) and 6-methyladenine (6MA) in modified and nonmodified DNAs from B. subtilis and B. subtilis phage SPP1 were determined. Nonmodified SPP1.0 DNA contains about 15 5~C residues/molecule. Each modified SPP1 .R DNA molecule carries 190 modification specific methyl gr

Restriction and modification in Bacillus
Restriction and modification in Bacillus subtilis: Expression of the cloned methyltransferase gene from B. subtilis phage SPR in E. coli and B. subtilis
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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h