Detection and characterization of naturally occuring plasmids in Bacillus subtilis
✍ Scribed by Le Hégarat, J.-C. ;Anagnostopoulos, C.
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 764 KB
- Volume
- 157
- Category
- Article
- ISSN
- 0026-8925
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Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inacti
Directly repeated pBR322 sequences 3.7-3.8 kb long recombine with a frequency of about 10% per generation when carried on plasmids related to pC194 and 0.01% per generation when carried on the Bacillus subtilis chromosome. Recombination is therefore 1,000 times more efficient in plasmids than in the
Using precise excision as a model system, we have quantified the effect of direct repeats, inverted repeats and the size of the spacer between the repeats in the process of deletion formation in Bacillus subtilis. Both in the presence and absence of inverted repeats, the frequency of precise excisio