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Recombination between repeated DNA sequences occurs more often in plasmids than in the chromosome of Bacillus subtilis

✍ Scribed by Niaudet, B. ;Jannière, L. ;Ehrlich, S. D.

Publisher
Springer
Year
1984
Tongue
English
Weight
916 KB
Volume
197
Category
Article
ISSN
0026-8925

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✦ Synopsis

Directly repeated pBR322 sequences 3.7-3.8 kb long recombine with a frequency of about 10% per generation when carried on plasmids related to pC194 and 0.01% per generation when carried on the Bacillus subtilis chromosome. Recombination is therefore 1,000 times more efficient in plasmids than in the chromosome of this organism.

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✍ Gryczan, Thomas ;Contente, Sara ;Dubnau, David 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 926 KB

The difficulty experienced in the shotgun cloning of chromosomal DNA on plasmid vectors in Bacillus subtilis is analyzed and an explanation for this difficulty is offered based on an inherent property of competent cells which imposes a requirement of plasmid multimers in transformation of plasmid-fr