Whole cells of Kluyvera citropbila were immobilized in polyacrylamide gel. The penicillin acylase activity of immobilized whole cells was 60%-70% of native cells. When the immobilized cells were continuously cultivated for 40 h in an aerated fermentor containing peptone medium and were treated with
Enhancing enzymatic activity of penicillin G acylase by coexpressingpcmgene
β Scribed by Tianwen Wang; Hu Zhu; Xingyuan Ma; Zhuoya Fei; Yushu Ma; Dongzhi Wei
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 141 KB
- Volume
- 72
- Category
- Article
- ISSN
- 1432-0614
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## Abstract Five different ionic liquids, based on dialkylimidazolium cations associated with perfluorinated and bis{(trifluoromethyl)sulfonyl}imide anions, were used to investigate the scope and limitations of these new solvents as media for penicillin G acylaseβcatalyzed reactions. Deactivation o